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JoVE Journal
Developmental Biology

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Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
 

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Article DOI: 10.3791/60233-v 09:08 min September 18th, 2019
September 18th, 2019

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Summary

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

Tags

Keywords: Blood-brain Barrier Drosophila Melanogaster Embryo Collection Barrier Permeability Apple Juice Agar PbTx Dechorionation Live Tissue Imaging
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