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Analysis of Hematopoietic Stem Progenitor Cell Metabolism
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Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

DOI:
10.3791/60234-v

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12:20 min

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November 09, 2019

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, , , ,
1Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

Chapters

  • 00:05Title
  • 01:13Preparation of Reagents on the Day Prior to the Assay
  • 02:09Day of the Assay
  • 07:10Mitochondrial Respiration and Glycolysis Assays of LSK HSPCs
  • 09:21Results: Analysis of Hematopoietic Stem Progenitor Cell Metabolism
  • 11:37Conclusion

Summary

Automatic Translation

Automatic Translation

Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.

Tags

Hematopoietic Stem CellsHematopoietic Progenitor CellsMetabolic RegulationMitochondrial RespirationGlycolysisExtracellular Flux AnalysisMurine Bone MarrowDensity GradientMononucleated Cells

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