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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
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JoVE Journal Biology
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

DOI:
10.3791/60255-v

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07:14 min

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September 20, 2019

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, ,
1Department of Biology and Biotechnology,Worcester Polytechnic Institute

Chapters

  • 00:04Title
  • 00:36Live Cell Imaging Preparation
  • 02:06Microscope Setup
  • 03:55Time-Lapse Image Analysis
  • 05:09Results: Representative Time-Lapse Imaging of Mitotic Fidelity
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to assess the dynamics of spindle formation and mitotic progression. Our application of time-lapse imaging enables the user to identify cells at various stages of mitosis, track and identify mitotic defects, and analyze spindle dynamics and mitotic cell fate upon exposure to anti-mitotic drugs.

Tags

Live Cell ImagingMetaphaseMitotic Spindle PerturbationsPhototoxicityTrypsinCell CultureMitotic DrugEpifluorescence MicroscopeEnvironmental ChamberNumerical ApertureFluorescencePhase ContrastBright Field Imaging

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