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マウスマンモスフィア培養における自己再生の定量
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Quantification of Self-renewal in Murine Mammosphere Cultures
DOI:

07:40 min

November 26, 2019

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Chapters

  • 00:04Title
  • 01:09Murine Mammary Gland Collection and Digestion
  • 01:52Primary Murine Mammary Epithelial Cell (MEC) Isolation
  • 03:15Serial Mammosphere Re-Plating
  • 04:12Sphere Enumeration by Digital Image Analysis (DIA)
  • 05:45Cumulative Growth Curve Calculation
  • 06:38Results: Representative Sphere and Cell Growth Numbers and Curves
  • 07:05Conclusion

Summary

Automatic Translation

ここでは、インビトロマンモスフィア自己再生定量アッセイの実施と解釈について説明する。

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