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Neuroscience

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JoVE Journal Neuroscience
Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons
 

Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons

Article DOI: 10.3791/60287-v • 08:23 min • September 25th, 2019
September 25th, 2019 •
1, 1, 1, 1, 1, 1
1National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Here, we describe the method we employed to image highly motile dendritic filopodia in a live preparation of the Drosophila larval brain, and the protocol we developed to quantify time-lapse 3D imaging datasets for quantitative assessments of dendrite dynamics in developing neurons.

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Keywords: Time-lapse Imaging Dendritic Branch Dynamics Drosophila Neurons 3D Image Annotation Branch Terminal Tracking Dendrite Morphogenesis Larval Brain Dissection External Saline Solution Two-photon Microscopy Drift Correction
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