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Et semi-High-gennemløb Imaging metode og data visualisering Toolkit til at analysere C. elegans embryonale udvikling
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1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine,University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program,University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research,National Institutes of Health, 6Department of Biology,San Diego State University, 7Developmental and Stem Cell Biology Graduate Program,University of California, San Francisco
Chapters
- 00:04Title
- 01:01C. elegans Embryo Semi-High-Throughput Imaging Preparation
- 03:15Automated Cropping
- 04:12Visualization
- 05:11Results: Representative High-Content Imaging of C. elegans Embryogenesis
- 05:56Conclusion
Dette arbejde beskriver en semi-High-gennemløb protokol, der tillader samtidige 3D time-lapse Imaging af embryogenese i 80 – 100 C. elegans embryoner i en enkelt overnight kørsel. Derudover er billedbehandling og visualiseringsværktøjer inkluderet for at strømline dataanalyse. Kombinationen af disse metoder med brugerdefinerede reporter stammer muliggør detaljeret monitorering af embryogenese.
