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Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

DOI:
10.3791/60400-v

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08:49 min

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March 18, 2020

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1Department of Veterinary and Biomedical Sciences,University of Minnesota

Chapters

  • 00:04Introduction
  • 01:09Stimulating Rhesus PBMCs with Plate-bound Anti-CD3 and Soluble Anti-CD28
  • 02:47Transduction of Activated Rhesus PBMCs
  • 05:16Expansion of the Transduced Cells
  • 06:45Results: Analysis of Transduced and Expanded Cells
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

We outline a 9-day protocol for the transduction and expansion of rhesus macaque peripheral blood mononuclear cells which yields cells with excellent co-expression of the genes of interest in sufficient number for infusion studies of cell efficacy.

Tags

T-cellsTransductionExpansionCentral Memory PhenotypeChimeric Antigen ReceptorCXCR5Lymphoid FolliclesImmunotherapyRhesusHIVPBMCCell CultureCell CountingAnti-CD3Anti-CD28Virus Transduction

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