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Isolering og kultur av nervus, Trochlear, og spinal motor neurons fra prenatal ISLMN: GFP transgene mus
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1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Chapters
- 00:05Title
- 01:17Ventral Midbrain and Spinal Cord Dissection
- 06:10Tissue Dissociation
- 07:23Culture of Purified Primary Motor Neurons
- 07:59Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
- 09:34Conclusion
Dette arbeidet presenterer en protokoll for å gi homogene cellekulturer av primære nervus, trochlear, og spinal motor neurons. Disse kulturene kan brukes til sammenlignende analyser av morfologiske, cellulære, molekylære, og elektrofysiologisk karakteristikker av øye og spinal motor neurons.
