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Fluorescensaktivert cellesortering for isolering av sklerractiniske cellepopulasjoner
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JoVE Journal Biology
Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

Fluorescensaktivert cellesortering for isolering av sklerractiniske cellepopulasjoner

DOI:
10.3791/60446-v

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04:32 min

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May 31, 2020

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, , ,
1Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science,University of Miami, 2Department of Biology,University of Miami, 3The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center,Ben Gurion University of the Negev

Chapters

  • 00:04Introduction
  • 00:36Fluorescence-Activated Cell Sorting (FACS) Gating Strategy Setup and Analysis
  • 01:49FACS Sorting and Cell Collection
  • 02:55Results: Representative Live Coral Cell Gating
  • 03:59Conclusion

Summary

Automatic Translation

Automatic Translation

Koraller skaper biomangfoldige økosystemer som er viktige for både mennesker og marine organismer. Men vi forstår fortsatt ikke det fulle potensialet og funksjonen til mange korallceller. Her presenterer vi en protokoll utviklet for isolasjon, merking og separasjon av steinete korallcellepopulasjoner.

Tags

Flow CytometryFluorescence-activated Cell SortingCoral Cell IsolationCell Population IdentificationCell StainingGating StrategyCell CollectionPurity CheckIn Vitro Studies

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