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Developmental Biology
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毛因神经发生期间诺奇信号动力学的实时生物发光成像
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JoVE Journal
Developmental Biology
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Please note that all translations are automatically generated.
Click here for the English version.
毛因神经发生期间诺奇信号动力学的实时生物发光成像
DOI:
10.3791/60455-v
•
10:25 min
•
December 12, 2019
•
Hiromi Shimojo
,
Ryoichiro Kageyama
2,3,4
1
Institute for Frontier Life and Medical Sciences
,
Kyoto University
,
2
Institute for Integrated Cell-Material Sciences (iCeMS)
,
Kyoto University
,
3
Kyoto University Graduate School of Medicine
,
4
Kyoto University Graduate School of Biostudies
Chapters
00:04
Title
00:46
In Utero Electroporation
02:29
Neural Stem/Progenitor Cell (NPC) Dissociation Culture
05:16
Luciferase Reporter Expression Visualization
05:52
Slice Culture Preparation
07:31
Slice Culture Luciferase Reporter Visualization
08:09
Results: Representative DII1 Protein Visualization and Dynamic Expression Analysis in Cortical Slice Cultures
09:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
神经干细胞/祖细胞表现出Notch信号分量的各种表达动力学,导致细胞事件的不同结果。这种动态表达可以通过实时监测,而不是静态分析来显示,使用高度敏感的生物发光成像系统,能够可视化基因表达的快速变化。
Tags
Bioluminescence Imaging
Notch Signaling
Neurogenesis
Neural Progenitor Cells
Dll1 Reporter
In Vivo Imaging
Real-time Gene Expression
Cell Proliferation
Cell Differentiation
Embryonic Development
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