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Kultur, Manipulation, og ortotoptransplantation af mus blære Tumor organoider
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
DOI:

08:43 min

January 31, 2020

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Chapters

  • 00:04Title
  • 00:50In Vitro Culture of Bladder Tumor Organoids
  • 03:50Genetic Manipulation of Bladder Organoids via Lentivirus-mediated Transduction
  • 04:36Orthotopic Transplantation of Bladder Organoids
  • 07:06Results: Culture and Transplantation of Bladder Tumor Organoids
  • 08:01Conclusion

Summary

Automatic Translation

Denne protokol indeholder detaljerede eksperimentelle skridt til at etablere en tre-dimensionel in vitro kultur af blæretumor organoider stammer fra kræftfremkaldende-induceret murine blærekræft. Kultur metoder, herunder passaging, genteknologi, og ortotoptransplantation af tumor organoider er beskrevet.

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