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En menneskelig 3D ekstracellulær matrix-Adipocyte kultur model for at studere matrix-celle metaboliske Crosstalk
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
DOI:

09:04 min

November 07, 2019

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Chapters

  • 00:05Title
  • 01:20Adipose Tissue Procurement and Preadipocyte Isolation
  • 02:51Adipose Tissue ECM Preparation
  • 05:08ECM-Adipocyte Preparation
  • 07:04Results: Genotypic and Phenotypic Characterization of In vitro Differentiated Adipocytes
  • 08:36Conclusion

Summary

Automatic Translation

Vi beskriver en 3D menneskelige ekstracellulære matrix-adipocyte in vitro kultur system, der tillader dissektion af rollerne af matrix og adipocytter i at bidrage til fedtvæv metabolisk phenotype.

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