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In vitro stimulation og visualisering af ekstracellulær fælde frigivelse i differentierede humane monocytter-afledte makrofager
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
DOI:

08:08 min

November 01, 2019

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Chapters

  • 00:05Title
  • 01:35Polarization of HMDM (Human Monocyte-derived Macrophages)
  • 02:36Stimulation of HMDM to Induce MET (Macrophage Extracellular Traps) Release
  • 04:20Visualization of MET in Live Cell Culture
  • 05:46Results: Morphological Changes of Differentially Polarized HMDMs and Release of METs Following Stimulation
  • 07:17Conclusion

Summary

Automatic Translation

Præsenteret her er en protokol til påvisning af makrofag ekstrellulær fælde (MET) produktion i levende cellekultur ved hjælp af mikroskopi og fluorescens farvning. Denne protokol kan udvides yderligere til at undersøge specifikke MARKEDSført protein markører ved immunofluorescens farvning.

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