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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
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Cancer Research
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JoVE Journal Cancer Research
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

DOI:
10.3791/60549-v

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12:13 min

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November 19, 2019

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, , , ,
1Department of Biochemistry and Molecular Biology,Augusta University, 2Department of Urology,Veterans Affairs Medical Center and University of California, San Francisco, 3Department of Pathology,Veterans Affairs Medical Center and University of California, San Francisco

Chapters

  • 00:05Title
  • 01:11Isolating Serum-derived EVs (Extracellular Vesicles)
  • 02:14Library Preparation for Small RNA Sequencing: Generate Adapter-Ligated cDNA
  • 06:08Library Preparation for Small RNA Sequencing: Purify and Perform Quality Control for the Ligated cDNA
  • 09:39Results: Library Preparation, Quality Check, and Run Metrics for Sequencing Small Non-coding RNA
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

Therapy resistance often develops in patients with advanced prostate cancer, and in some cases, cancer progresses to a lethal subtype called neuroendocrine prostate cancer. Assessing the small non-coding RNA-mediated molecular changes that facilitate this transition would allow better disease stratification and identification of causal mechanisms that lead to development of neuroendocrine prostate cancer.

Tags

Keywords: RNA SequencingSerum-derived ExosomesCastration-resistant Prostate CancerNext-generation SequencingCDNA Library PreparationRNA IsolationExosome IsolationProstate Cancer Biology

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