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使用嘧啶类似物5-碘-2′-脱氧尿苷（IdU）和细胞周期标志物在质谱细胞术平台中建立细胞周期期

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Genetics

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JoVE Journal Genetics

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Please note that all translations are automatically generated. Click here for the English version.

使用嘧啶类似物5-碘-2′-脱氧尿苷（IdU）和细胞周期标志物在质谱细胞术平台中建立细胞周期期

DOI:

10.3791/60556-v

•

08:37 min

•

October 22, 2021

•

Raymond D Devine, Gregory K Behbehani

¹Ohio State Comprehensive Cancer Center, Division of Hematology,The Ohio State University

Chapters

00:04Introduction
00:515‐Iodo‐2’‐Deoxyuridine (IdU) Incorporation and Sample Preservation
02:28Data Analysis
06:27Results: Representative Cell Cycle Phase Establishment
07:55Conclusion

Summary

Automatic Translation

该协议调整细胞周期测量以用于质质细胞术平台。凭借质谱细胞术的多参数功能，直接测量碘掺入可以鉴定S期细胞，而细胞内循环标记物可以在一系列实验条件下表征每个细胞周期状态。

Tags

Cell Cycle Mass Cytometry 5-Iodo-2′-Deoxyuridine (IdU)Cell Cycle Markers Acute Myeloid Leukemia Stem And Progenitor Cells Cell Growth Cell Proliferation Live/dead Stain Paraformaldehyde Cell Staining Medium DMSO Iridium

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