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冠状血管生成体模型
JoVE Journal
Medicine
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JoVE Journal Medicine
In Vitro Model of Coronary Angiogenesis
DOI:

08:03 min

March 10, 2020

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Chapters

  • 00:04Introduction
  • 00:38Embryo Harvesting
  • 01:28E11.5 Embryo Heart Isolation
  • 02:14Sinus Venosus (SV) and Ventricle Isolation
  • 03:09SV and Whole Ventricle Culture
  • 04:09Vascular Endothelial Growth Factor-A (VEGF-A) Treatment
  • 04:54Fixation, Immunostaining, Mounting, and Analysis
  • 06:22Results: Representative Coronary Angiogenesis Evaluation
  • 07:12Conclusion

Summary

Automatic Translation

冠状血管生成体外模型可用于发现冠状血管生成细胞和分子机制。正月线和内卡组织体外外培养物在响应VEGF-A时表现出强劲的生长,并表现出与体内类似的COUP-TFII表达模式。

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