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Cotocação e gota lipídica manchando de enocitos nas larvas de Drosophila
JoVE Journal
Biology
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JoVE Journal Biology
Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
DOI:

05:37 min

December 28, 2019

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Chapters

  • 00:04Title
  • 00:32Egg Laying
  • 01:13Starvation Treatment
  • 02:10Oenocyte Dissection
  • 03:10Lipid Droplet Staining
  • 04:02Mounting and Imaging
  • 04:41Results: Representative Lipid Droplet Staining
  • 05:06Conclusion

Summary

Automatic Translation

Aqui são apresentados métodos detalhados para a dissecação e mancha de gotícula lipídica de estenocitos em larvas de Drosophila usando BODIPY 493/503, um corante fluorescente específico da gotícula lipídica.

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