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Developmental Biology

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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
 

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Article DOI: 10.3791/60647-v 08:30 min January 7th, 2020
January 7th, 2020

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Summary

Here we presented a multiplexed single cell mRNA sequencing method to profile gene expression in mouse embryonic tissues. The droplet-based single cell mRNA sequencing (scRNA-Seq) method in combination with multiplexing strategies can profile single cells from multiple samples simultaneously, which significantly reduces reagent costs and minimizes experimental batch effects.

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Keywords: Single Cell MRNA Sequencing Multiplexing Cell Isolation Cell Preparation Cell Counting Anchor Barcode Co-anchor Barcode Cell Filtering Chip Assembly Cell Solution Preparation Gel Bead Addition Partitioning Oil Chromium Controller Single Cell Analysis
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