/

/

Ex Vivo 加压希马卡毛细管- 功能研究准备

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study

Please note that all translations are automatically generated. Click here for the English version.

Ex Vivo 加压希马卡毛细管- 功能研究准备

DOI:

10.3791/60676-v

•

09:15 min

•

December 18, 2019

•

Amanda C. Rosehart, Abbie C. Johnson, Fabrice Dabertrand³

¹Department of Anesthesiology,University of Colorado Anschutz Medical Campus, ²Department of Neurological Sciences,University of Vermont Larner College of Medicine, ³Department of Pharmacology,University of Colorado Anschutz Medical Campus

Chapters

00:04Title
00:32Hippocampus Dissection and Isolation
02:22Hippocampal Arteriole Isolation
03:10Hippocampal Capillary-Parenchymal Arteriole Cannulation
04:27Pressure Myography
05:55Capillary End Focal Stimulation
07:27Results: Representative Effects of Focal Stimulation on Upstream Arteriolar Diameter Dilation
08:42Conclusion

Summary

Automatic Translation

本手稿详细介绍了如何从小鼠大脑中分离海马动脉和毛细血管，以及如何为压力的焦疗、免疫荧光、生物化学和分子研究加压它们。

Tags

Keywords: Ex Vivo Hippocampal Capillary-parenchymal Arteriole Functional Study Dissection Mouse Brain Hippocampus Arteriole Isolation Transverse Artery MOPS Buffered Saline Cerebellum Thalamus Corpus Callosum Cerebrum

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

突触改变老化过程中淀粉样蛋白病理研究从大鼠和转基因小鼠海马急性片的制备

神经元形态的研究方法：<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔

使用超声波血脑屏障破坏和锰增强MRI的脑功能成像

鼠标后脑<em>前体内</em>企业文化来研究面部Branchiomotor神经元迁移

同时<em>体外</em两个由视网膜>功能测试<em>在体内</em>电图系统

在脑片恐惧电路体外光遗传学解剖

富氧运动器械中慢性硅探针的植入及海马位细胞的记录

为电子生理记录准备急性人类河马切片

大鼠坐骨神经用于离体神经生理学的制备

离体基于OCT的人供体眼睛多模态成像用于研究年龄相关性黄斑变性

Read Article