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Trinvis doseringprotokol for øget overførselstid i label-free impedansbaserede GPCR-analyser
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1Institute of Analytical Chemistry, Chemo- and Biosensors,University of Regensburg, 2Institute of Pharmacy,University of Regensburg, 3Department of Chemistry and Pharmacy,Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT
Chapters
- 00:04Introduction
- 01:01Electrode Array Cell Seeding and Equilibration
- 01:41Cell Equilibration Monitoring and Serial Agonist Addition
- 03:06Data Export and Analysis
- 03:52Results: Representative Impedance-Based GPCR Analyses
- 05:15Conclusion
Denne protokol viser real-time registrering af fuld dosis-respons relationer for agonist-induceret GPCR aktivering fra en enkelt celle lag dyrket på en enkelt mikroelektrode ved hjælp af label-fri impedans målinger. Den nye doseringsordning øger gennemløb betydeligt uden tab i tidsløsning.
