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植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定

JoVE Journal
Genetics

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JoVE Journal Genetics

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Please note that all translations are automatically generated. Click here for the English version.

植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定

DOI:

10.3791/60697-v

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10:19 min

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February 03, 2020

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Jinxia Shi, Yijuan Jia, Di Fang, Shidan He, Peng Zhang², Yushuang Guo, Yongli Qiao

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences,Shanghai Normal University, ²College of Agriculture,Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation,Guizhou Institute of Tobacco Science

Chapters

00:04Introduction
00:26Preparation of N. benthamiana 16c Plants
02:08Preparation of Agrobacterium Culture for Infiltration
03:44Co-infiltration N. benthamiana Leaves
04:26GFP Imaging Analysis
04:47Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
08:08Results: Infiltrated N. benthamiana 16c Leaves
09:24Conclusion

Summary

Automatic Translation

在这里，我们提出了一种经过改良的筛选方法，该方法可广泛用于快速筛选植物病原体中的RNA沉默抑制剂。

Tags

RNA Silencing Suppressors Plant Pathogens Screening Assay Nicotiana Benthamiana Systemic RNA Silencing Local RNA Silencing Agrobacterium LB Medium Antibiotics MES Acetosyringone

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