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Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

JoVE Journal
Genetics
Author Produced

This content is Free Access.

JoVE Journal Genetics

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

DOI:

10.3791/60749-v

•

12:32 min

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December 14, 2019

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Tanita Wein*¹, Fenna T. Stücker*¹, Nils F. Hülter, Tal Dagan

¹Institute of Microbiology,Kiel University

Chapters

00:00Title
01:53Monitoring Plasmid-carrying Bacteria under Various Conditions over Time
10:14Results
11:22Conclusion

Summary

Automatic Translation

Our experimental approach provides a strategy to follow plasmid abundance and antibiotic resistance over time in bacterial populations.

Tags

Plasmid Antibiotic Resistance Lateral Gene Transfer PCR Isothermal Assembly E. Coli MG1655 Experimental Evolution Replica Plating 96-well Plate

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