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在生物标志物分析临床试验场单次抽血时，准备外周血单核细胞颗粒和血浆
JoVE Journal
Medicine
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JoVE Journal Medicine
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
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在生物标志物分析临床试验场单次抽血时，准备外周血单核细胞颗粒和血浆

DOI:
10.3791/60776-v

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07:40 min

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March 20, 2021

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, , , , , , ,
1Translational Medicine, R&D Oncology,AstraZeneca, 2Acerta Pharma, AstraZeneca Group, 3Translational Medicine, R&D Oncology,AstraZeneca

Chapters

  • 00:05Introduction
  • 01:08Whole Blood Irradiation for DNA Damage Response (DDR) Biomarker Expression Analysis
  • 03:35PBMC and Plasma Freezing
  • 04:45Western Blot Analysis
  • 05:43Results: Representative DNA Damage Assessment
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

本协议详细介绍了可用于转化生物标志物分析的临床试验现场高质量PBMC和血浆生物采样剂的临床可实施制备。

Tags

Keywords: PBMCPlasmaBlood DrawBiomarker AnalysisClinical TrialPre-analytical VariabilityPharmacokineticsPharmacodynamicsDNA Damage ResponseRadiationCentrifugationCell SeparationBuffy Coat

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