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Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
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Bioengineering
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JoVE Journal Bioengineering
Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

DOI:
10.3791/60781-v

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09:35 min

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June 16, 2020

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, ,
1Department of Biomedical Engineering,The George Washington University

Chapters

  • 00:04Introduction
  • 00:29Optical Mapping System Setup
  • 01:16Slicing Protocol
  • 02:41Static Slice Culture
  • 03:22Optical Mapping
  • 05:01Data Processing
  • 07:24Results: Representative Transmembrane Potential and Calcium Transient Mapping
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the procedure for sectioning and culturing human cardiac slices for preclinical drug testing and details the use of optical mapping for recording transmembrane voltage and intracellular calcium signals simultaneously from these slices.

Tags

Optical MappingCardiac ElectrophysiologyHuman Organotypic Cardiac SlicesVoltage DyeCalcium DyeAction PotentialsIntracellular Calcium TransientsPerfusion SystemVibratomeCardioplegiaTyrode’s Recovery SolutionPreclinical Physiological Drug Testing

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