/
/
En intravital mikroskopi-baseret tilgang til vurdering af intestinal permeabilitet og epitelcelle shedding performance
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 01:07Intravital Microscopy Preparation
- 02:19Surgical Preparation
- 03:06Intestinal Mouse Mucosa Staining
- 03:54Intravital Microscopy Imaging
- 05:44Results: Representative Epithelial Integrity Analysis
- 06:51Conclusion
Drage fordel af intravital mikroskopi, den metode, der præsenteres her gør det muligt real-time visualisering af intestinal epitelcelle udgydelse i levende dyr. Derfor, topisk plettet tarmslimhinden (acriflavine og rhodamineB-dextran) af bedøvede mus er afbildet op til encellet opløsning ved hjælp af konfokal mikroskopi.
Tags
Keywords:
Intravital MicroscopyIntestinal PermeabilityEpithelial Cell SheddingReal-time VisualizationSingle-cell ResolutionPara And Transcellular PermeabilitySurgical PreparationConfocal Laser Scanning MicroscopeImage AcquisitionSequential AcquisitionAnesthetized MouseIntestinal LumenExteriorized Intestinal SegmentIntestinal Mucosa SurfaceAcriflavine Staining
