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Journal
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Biology
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LarvaSPA,一种用于长期延时成像的安装
果蝇
幼虫的方法
JoVE Journal
Biology
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JoVE Journal
Biology
LarvaSPA, A Method for Mounting
Drosophila
Larva for Long-Term Time-Lapse Imaging
Please note that all translations are automatically generated.
Click here for the English version.
LarvaSPA,一种用于长期延时成像的安装
果蝇
幼虫的方法
DOI:
10.3791/60792-v
•
08:55 min
•
February 27, 2020
•
Hui Ji
,
Chun Han
1
Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics
,
Cornell University
Chapters
00:04
Introduction
01:20
Making Imaging Chamber
01:40
Making PDMS Cuboids
03:53
Mounting Larvae for Long-term Time-lapse Imaging
06:47
Recovery of Imaging Chamber and PDMS Cuboids
07:20
Results: Dendrite Dynamics and Dendrite Degeneration
07:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种在完整的活体动物中安装
果蝇
幼虫以实现超过10小时的不间断延时成像的方法。该方法可用于成像许多靠近幼虫体壁的生物过程。
Tags
LarvaSPA
Drosophila Larva
Live Imaging
Time-lapse Imaging
Peripheral Sensory Neurons
Dendrite Development
Dendrite Degeneration
PDMS Cuboids
Aluminum Block
Cover Slip
UV Glue
Packaging Tape
PDMS Mixture
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