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LarvaSPA，一种用于长期延时成像的安装果蝇幼虫的方法
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JoVE Journal Biology
LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging
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LarvaSPA，一种用于长期延时成像的安装果蝇幼虫的方法

DOI:
10.3791/60792-v

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08:55 min

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February 27, 2020

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,
1Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics,Cornell University

Chapters

  • 00:04Introduction
  • 01:20Making Imaging Chamber
  • 01:40Making PDMS Cuboids
  • 03:53Mounting Larvae for Long-term Time-lapse Imaging
  • 06:47Recovery of Imaging Chamber and PDMS Cuboids
  • 07:20Results: Dendrite Dynamics and Dendrite Degeneration
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种在完整的活体动物中安装果蝇幼虫以实现超过10小时的不间断延时成像的方法。该方法可用于成像许多靠近幼虫体壁的生物过程。

Tags

LarvaSPADrosophila LarvaLive ImagingTime-lapse ImagingPeripheral Sensory NeuronsDendrite DevelopmentDendrite DegenerationPDMS CuboidsAluminum BlockCover SlipUV GluePackaging TapePDMS Mixture

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