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LarvaSPA, en metode for montering drosophila larve for langsiktig time-lapse imaging
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JoVE Journal Biology
LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging

LarvaSPA, en metode for montering drosophila larve for langsiktig time-lapse imaging

DOI:
10.3791/60792-v

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08:55 min

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February 27, 2020

•

,
1Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics,Cornell University

Chapters

  • 00:04Introduction
  • 01:20Making Imaging Chamber
  • 01:40Making PDMS Cuboids
  • 03:53Mounting Larvae for Long-term Time-lapse Imaging
  • 06:47Recovery of Imaging Chamber and PDMS Cuboids
  • 07:20Results: Dendrite Dynamics and Dendrite Degeneration
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver en metode for montering av Drosophila larver for å oppnå mer enn 10 timer uavbrutt time-lapse imaging hos intakte levende dyr. Denne metoden kan brukes til å bilde mange biologiske prosesser nær larval kroppen veggen.

Tags

LarvaSPADrosophila LarvaLive ImagingTime-lapse ImagingPeripheral Sensory NeuronsDendrite DevelopmentDendrite DegenerationPDMS CuboidsAluminum BlockCover SlipUV GluePackaging TapePDMS Mixture

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