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냉동 조직 섹션의 다스펙트럼 형광 이미징을위한 신속한 방법
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
DOI:

07:50 min

March 30, 2020

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Chapters

  • 00:05Introduction
  • 00:39Antibody and Fluorophore Selecting and Staining
  • 01:52Spectral Library Preparation
  • 02:59Multispectral Imaging
  • 03:54Spectral Unmixing
  • 04:37Analyzing Multispectral Images via Cell Segmentation and Phenotyping
  • 05:52Results: Representative Multispectral Imaging and Cell Quantification
  • 07:07Conclusion

Summary

Automatic Translation

우리는 냉동 된 조직에 다중 스펙트럼 이미징을 수행하는 빠른 염색 방법을 설명합니다.

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