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使用冷冻胚胎进行转基因小鼠高效生产
JoVE Journal
Genetics
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JoVE Journal Genetics
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
DOI:

06:46 min

April 02, 2020

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Chapters

  • 00:05Introduction
  • 00:35In Vitro Fertilization
  • 02:23Embryo Cryopreservation
  • 03:13Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
  • 04:19CRISPR Electroporation
  • 05:09Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
  • 06:09Conclusion

Summary

Automatic Translation

在这里,我们提出了一种用于冷冻保存单细胞胚胎的改良方法,以及一种用于使用冷冻解冻胚胎和电穿孔以有效生成转基因小鼠的协议。

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