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Journal
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Genetics
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使用冷冻胚胎进行转基因小鼠高效生产
JoVE Journal
Genetics
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JoVE Journal
Genetics
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Please note that all translations are automatically generated.
Click here for the English version.
使用冷冻胚胎进行转基因小鼠高效生产
DOI:
10.3791/60808-v
•
06:46 min
•
April 02, 2020
•
Hirofumi Nishizono*
1,2,3
,
Mohamed Darwish*
4,5
,
Hideki Uosaki
7
,
Nanami Masuyama
9,10
,
Motoaki Seki
11
,
Hiroyuki Abe
,
Nozomu Yachie
9,10,12,13
,
Ryohei Yasuda
1
Max Planck Florida Institute for Neuroscience
,
2
Life Science Research Center
,
University of Toyama
,
3
Department of Biochemical Engineering, Graduate School of Science and Engineering
,
Yamagata University
,
4
Graduate School of Innovative Life Science
,
University of Toyama
,
5
Department of Biochemistry, Faculty of Pharmacy
,
Cairo University
,
6
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
7
Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology
,
Jichi Medical University
,
8
Synthetic Biology Division, Research Center for Advanced Science and Technology
,
University of Tokyo
,
9
Institute for Advanced Biosciences
,
Keio University
,
10
Graduate School of Media and Governance
,
Keio University
,
11
Department of Molecular Oncology, Graduate School of Medicine
,
Chiba University
,
12
Department of Biological Sciences, School of Science
,
University of Tokyo
,
13
College of Arts and Sciences
,
University of Tokyo
Chapters
00:05
Introduction
00:35
In Vitro Fertilization
02:23
Embryo Cryopreservation
03:13
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
04:19
CRISPR Electroporation
05:09
Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
06:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种用于冷冻保存单细胞胚胎的改良方法,以及一种用于使用冷冻解冻胚胎和电穿孔以有效生成转基因小鼠的协议。
Tags
Genetically Modified Mice
Freeze-thawed Embryos
In Vitro Fertilization
Super Ovulation
Sperm Capacitation
Cryopreservation
Embryo Electroporation
High-efficiency
Low Mosaic Rate
Protocol
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