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通过可切换原卟啉IX依赖性原位活性氧物种光生成刺激小鼠皮肤中的干细胞壁龛和组织再生
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JoVE Journal
Biology
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
Please note that all translations are automatically generated.
Click here for the English version.
通过可切换原卟啉IX依赖性原位活性氧物种光生成刺激小鼠皮肤中的干细胞壁龛和组织再生
DOI:
10.3791/60859-v
•
10:05 min
•
May 08, 2020
•
Jesús Espada
2
,
Elisa Carrasco
,
María I. Calvo-Sánchez
4
,
Sandra Fernández-Martos
,
Juan José Montoya
1
Ramón y Cajal Institute for Health Research (IRYCIS)
,
Ramón y Cajal University Hospital
,
2
Centro Integrativo de Biología y Química Aplicada (CIBQA)
,
Universidad Bernardo O’Higgins
,
3
Department of Molecular Biology, Faculty of Sciences
,
Universidad Autónoma de Madrid (UAM)
,
4
Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales
,
Universidad Francisco de Vitoria
,
5
Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine
,
Complutense University
Chapters
00:04
Introduction
01:26
Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation
02:58
Transient Nonlethal Reactive Oxygen Species (ROS) Production
04:37
BrdU Detection in Mouse Tail Skin
05:38
ROS Detection in Mouse Tail Skin
07:16
Results: Effects of Photodynamic mALA Treatment and Transient ROS Production
09:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议的目的是诱导小鼠皮肤中瞬时体内产生非致死水平的活性氧(ROS),进一步促进组织中的生理反应。
Tags
Stem Cell Niche
Reactive Oxygen Species
ROS
Protoporphyrin IX
Photodynamic Therapy
Skin
Hair Growth
Wound Healing
BrdU
Labeling
In Vivo
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