Journal
/
Medicine
/
Using a Chemical Biopsy for Graft Quality Assessment
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Using a Chemical Biopsy for Graft Quality Assessment

Using a Chemical Biopsy for Graft Quality Assessment

DOI:
10.3791/60946-v

•

05:00 min

•

June 17, 2020

•

, , , , , , , , ,
1Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy,Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, 2Multi Organ Transplant Program, Department of Surgery,Toronto General Hospital, University Health Network, 3Department of Transplantology and General Surgery, Collegium Medicum in Bydgoszcz, Antoni Jurasz University Hospital No. 1 in Bydgoszcz,Nicolaus Copernicus University in Torun, Bydgoszcz, Poland, 4Department of Medicine,Toronto General Hospital

Chapters

  • 00:04Introduction
  • 01:09Preparation of Probes and Extraction
  • 02:27Desorption
  • 03:02Results: Metabolomic and Lipidomic Analyses
  • 03:57Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents utilization of the chemical biopsy approach followed by comprehensive metabolomic and lipidomic analysis for quality assessment of kidney grafts allocated for transplantation.

Tags

Keywords: Chemical BiopsyOrgan TransplantationGraft Quality AssessmentSPME ProbesBiocompatible CoatingsTumor AnalysisPreconditioning MixtureSterilizationKidney CortexMetabolomic AnalysisLipidomic AnalysisLiquid ChromatographyMass Spectrometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60946?article_type=v

Mouse Models for Graft Arteriosclerosis
check_url/60946?article_type=v

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique
check_url/60946?article_type=v

Substernal Thyroid Biopsy Using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration
check_url/60946?article_type=v

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
check_url/60946?article_type=v

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
check_url/60946?article_type=v

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
check_url/60946?article_type=v

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome
check_url/60946?article_type=v

Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency
check_url/60946?article_type=v

A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
check_url/60946?article_type=v

Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg

Read Article