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Developmental Biology

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JoVE Journal Developmental Biology
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
 

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

Article DOI: 10.3791/60961-v • 08:05 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences

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Summary
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The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.

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Keywords: Drosophila Larval Pupal Testes Cell Division Live Imaging Fluorescence Microscopy Dissection Tissue Preparation Male Forceps Scalpel
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