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In situ Hybridization for Sipunculus nudus Coelomic Fluid
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In situ Hybridization for Sipunculus nudus Coelomic Fluid

In situ Hybridization for Sipunculus nudus Coelomic Fluid

DOI:
10.3791/61022-v

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07:35 min

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May 01, 2020

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, ,
1Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences,Huaqiao University

Chapters

  • 00:00Introduction
  • 00:44Collection of Coelomic Fluid
  • 01:33In situ Hybridization: Day 1
  • 03:20In situ Hybridization: Day 2
  • 05:10In situ Hybridization: Day 3
  • 06:17Results
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver en effektiv in situ hybridisering tilnærming for å oppdage mRNA uttrykksnivåer og romlige mønstre av målgener i Sipunculus nudus coelomic væske.

Tags

In Situ HybridizationSipunculus NudusCoelomic FluidMRNA ExpressionLncRNA ExpressionGene LocalizationFishery ResourceRNA ProbesProteinase KPFA FixationWash BuffersAntibody StainingAlkaline PhosphataseBCIP-NBT Staining

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