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Journal
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Immunology and Infection
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简化反向遗传学方法,恢复重组性轮状病毒表达报告蛋白
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Please note that all translations are automatically generated.
Click here for the English version.
简化反向遗传学方法,恢复重组性轮状病毒表达报告蛋白
DOI:
10.3791/61039-v
•
11:40 min
•
April 17, 2020
•
Asha A. Philip
,
Jin Dai
,
Sarah P. Katen
,
John T. Patton
1
Department of Biology
,
Indiana University
Chapters
00:04
Introduction
00:32
Generation of Recombinant Virus
04:00
Plaque Isolation of Recombinant Viruses
07:02
Gel Electrophoresis of Viral dsRNA
09:11
Results: Characteristics of the Recombinant Strains rSA11/wt and rSA11/NSP3-2A-3xFL-UnaG
10:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
从质粒DNA生成重组性轮状病毒为研究轮状病毒复制和发病机制以及发展轮状病毒表达载体和疫苗提供了重要工具。在这里,我们描述了一种简化的反向遗传学方法来产生重组性轮状病毒,包括表达荧光报告蛋白的菌株。
Tags
Reverse Genetics
Recombinant Rotavirus
Reporter Proteins
BHKT7 Cells
MA104 Cells
Transfection
Trypsin
Plasmid Mixture
Fluorescent Marker Proteins
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