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Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

JoVE Journal
Biology
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JoVE Journal Biology

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

DOI:

10.3791/61073-v

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09:17 min

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October 10, 2020

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Perwez Alam, Bryan D. Maliken, Malina J. Ivey, Shannon M. Jones, Onur Kanisicak

¹Department of Pathology and Laboratory Medicine, College of Medicine,University of Cincinnati

Chapters

00:00Introduction
01:14Preparation & Materials
02:49Heart Extraction
04:15Heart Digestion & Single-Cell Suspension
05:59Cardiomyocyte Plating & Transfection
08:13Results
08:50Conclusion

Summary

Automatic Translation

Here, we present a protocol for the isolation, transfection, and long-term culture of adult mouse and rat cardiomyocytes.

Tags

Keywords: Cardiomyocytes Adult Mouse And Rat Isolation Transfection Long-term Culture Proliferative Potential Gene Expression Cytokine Expression Injury Response Optimized Method Survival Rate Transfection Efficiency Surgical Instruments Profusion Apparatus Heparin Myocyte Buffer Enzyme Solution Anesthesia

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