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用于刺激骨质复变和分析骨骼重塑功能结果的实验室A芯片平台

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

Please note that all translations are automatically generated. Click here for the English version.

用于刺激骨质复变和分析骨骼重塑功能结果的实验室A芯片平台

DOI:

10.3791/61076-v

•

08:28 min

•

May 21, 2020

•

Sharon L. Truesdell, Estee L. George, Christopher C. Van Vranken, Marnie M. Saunders

¹Department of Biomedical Engineering,The University of Akron

Chapters

00:04Introduction
00:45PDMS Fabrication
02:15Chip Assembly and Sterilization
03:47Mechanical Loading Device Assembly
04:33Loading Experiment
06:23Results: Functional Activity and Effects of Mechanically Induced Strain
07:48Conclusion

Summary

Automatic Translation

在这里，我们提出了用于在芯片实验平台内分析骨骼重塑的协议。3D 打印机械装载装置可与平台配对，通过变形细胞基质诱导骨质异质异体转导。该平台还可用于量化骨细胞和成骨细胞（再吸收/形成）的骨骼重塑功能结果。

Tags

Keywords: Lab-on-a-chip Osteocyte Mechanotransduction Bone Remodeling PDMS Microfluidics Bone Cell Culture Mechanical Loading Osteoporosis Fracture Healing Periprosthetic Osteolysis

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