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Oppdagelsen av drivergener i kolorektal HT29-avledede kreftstammelignende tumorsfærer
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1Radiation Biology Research Center, Institute for Radiological Research,Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences,Chang Gung University, 3Department of Radiation Oncology,Chang Gung Memorial Hospital, 4Department of Laboratory Medicine,Mackay Memorial Hospital
Chapters
- 00:04Introduction
- 00:36Cell Culture and Tumorsphere Formation
- 01:53RNA Isolation
- 03:11RNAseq Profiling and Bioinformatics Analysis
- 03:45Driver Gene Selection
- 04:52Results: Representative Tumorsphere Protein and Gene Expression Analyses
- 06:09Conclusion
Presentert her er en protokoll for å oppdage de overuttrykte drivergenene som opprettholder de etablerte kreftstamlignende cellene avledet fra kolorektal HT29-celler. RNAseq med tilgjengelig bioinformatikk ble utført for å undersøke og screene genuttrykksnettverk for å belyse en potensiell mekanisme involvert i overlevelsen av målrettede tumorceller.
