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Immunology and Infection

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使用 iDISCO + 和光片荧光显微镜对脊椎淋巴血管和⁺排水进行三维成像

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO⁺ and Light Sheet Fluorescence Microscopy

Please note that all translations are automatically generated. Click here for the English version.

使用 iDISCO + 和光片荧光显微镜对脊椎淋巴血管和⁺排水进行三维成像

DOI:

10.3791/61099-v

•

10:05 min

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May 22, 2020

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Laurent Jacob*¹, Jose Brito*^1,2, Jean-Leon Thomas³

¹Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, ²Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, ³Department of Neurology,Yale University School of Medicine

Chapters

00:04Introduction
00:49Intra-Cisterna Magna (ICM) and Thoracolumbar (ThLb) Injection
02:22Perfusion and Tissue Dissection
03:34Whole Mount Vertebral Segment Immunolabeling-Enabled Imaging of Solvent-Cleared Organs-Plus (iDISCO⁺)
06:14Light Sheet Fluorescence Microscopy (LSFM) Imaging
08:02Results: Representative Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage
09:21Conclusion

Summary

Automatic Translation

提出了一种将组织清除与光片荧光显微镜（LSFM）相结合的协议，以获得收集脑脊液（CSF）和脊柱硬膜外液的淋巴血管和淋巴结（LNs）的三维和细胞分辨率图像。

Tags

3D Imaging Vertebral Lymphatic Vasculature Lymphatic Drainage IDISCO+ Clearing Light Sheet Fluorescence Microscopy Spinal Cord Meninges Epidural Space Draining Lymph Nodes Fluorescent Tracer Perfusion Anatomical Imaging

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