Journal
/
Bioengineering
/
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

DOI:
10.3791/61106-v

•

08:13 min

•

February 27, 2021

•

, , , ,
1Laboratoire Matière et Systèmes,Complexes MSC, UMR 7057, CNRS & University Paris Diderot, 2Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science,LVTS, INSERM, UMR 1148, 3Services de Biochimie et Médecine Nucléaire,Hôpital Avicenne Assistance Publique-Hôpitaux de Paris

Chapters

  • 00:05Introduction
  • 00:58Synthesis of Magnetic Nanoparticles
  • 02:37Culture and Magnetic Labeling of Stem Cells
  • 03:33Formation of Stem Cell-Spheroids
  • 04:51Quantification of Magnetic Nanoparticles Using a Vibrating Sample Magnetometer (VSM)
  • 05:46Results: VSM Measures Uptake and Biodegradation of Nanoparticles
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Iron oxide nanoparticles are synthesized via a nonaqueous sol gel procedure and coated with anionic short molecules or polymer. The use of magnetometry for monitoring the incorporation and biotransformations of magnetic nanoparticles inside human stem cells is demonstrated using a vibrating sample magnetometer (VSM).

Tags

Keywords: Magnetic NanoparticlesMagnetometryCellular IncorporationBiodegradationIron Oxide NanoparticlesSynthesisBiomedical ApplicationsMagnetismContaminationMagnetometerLiquid SamplesBiological SamplesIron(III) AcetylacetonateMicrowave ReactorNeodymium MagnetHydrochloric AcidCoating MoleculesHuman Mesenchymal Stem CellsRPMI Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61106?article_type=v

Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
check_url/61106?article_type=v

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
check_url/61106?article_type=v

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles
check_url/61106?article_type=v

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
check_url/61106?article_type=v

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
check_url/61106?article_type=v

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping
check_url/61106?article_type=v

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis
check_url/61106?article_type=v

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
check_url/61106?article_type=v

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
check_url/61106?article_type=v

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Read Article