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Brug af magnetometri til at overvåge cellulær inkorporering og efterfølgende bionedbrydning af kemisk syntetiserede jernoxidnanopartikler
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
DOI:

08:13 min

February 27, 2021

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Chapters

  • 00:05Introduction
  • 00:58Synthesis of Magnetic Nanoparticles
  • 02:37Culture and Magnetic Labeling of Stem Cells
  • 03:33Formation of Stem Cell-Spheroids
  • 04:51Quantification of Magnetic Nanoparticles Using a Vibrating Sample Magnetometer (VSM)
  • 05:46Results: VSM Measures Uptake and Biodegradation of Nanoparticles
  • 07:22Conclusion

Summary

Automatic Translation

Jernoxid nanopartikler syntetiseres via en nonaqueous sol gel procedure og belagt med anioniske korte molekyler eller polymer. Brugen af magnetometri til overvågning af inkorporering og biotransformationer af magnetiske nanopartikler i menneskelige stamceller demonstreres ved hjælp af et vibrerende prøvemagnetometer (VSM).

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