JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
从鼠心心室自由壁分离内膜细胞和冠状内皮细胞
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
Please note that all translations are automatically generated.
Click here for the English version.
从鼠心心室自由壁分离内膜细胞和冠状内皮细胞
DOI:
10.3791/61126-v
•
08:22 min
•
April 15, 2020
•
Alyssa Klein
2,3
,
Bethel Bayrau
2,3
,
Yifei Miao
2,3,4,5
,
Mingxia Gu
2,3,4,5
1
Department of Pediatrics, Division of Cardiology
,
Stanford School of Medicine
,
2
Vera Moulton Wall Center for Pulmonary Vascular Disease
,
Stanford School of Medicine
,
3
Stanford Cardiovascular Institute
,
Stanford School of Medicine
,
4
Division of Pulmonary Biology, Department of Pediatrics
,
Cincinnati Children’s Hospital Medical Center
,
5
Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:04
Introduction
01:11
Heart Isolation
02:34
Endocardial Endothelial Cell (EEC) Digestion
03:34
Coronary Endothelial Cell (CEC) Digestion
04:36
Endothelial Cell (EC) Sorting
06:56
Results: Representative qPCR Data CEC and EEC Isolation Verification
07:46
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们提出了一种方案,通过消化缓冲液中的连续组织消化、循环离心循环的细胞采集以及使用抗大鼠CD31微珠进行细胞纯化,从大鼠心脏分离内皮和冠状内皮细胞。
Tags
Endocardial Cells
Coronary Endothelial Cells
Heart
Isolation
Cell Type-specific
Gene Expression
Heart Diseases
Transcriptomics
Epigenetics
Sprague Dawley Rats
Ventricular Free Wall
Dissection
Digestion Buffer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/61126?article_type=v
视网膜色素上皮细胞的干细胞高效的推导
check_url/61126?article_type=v
隔离和乳鼠心肌细胞冷冻保存
check_url/61126?article_type=v
从脑室周围地区的成年大鼠和人类脊髓神经分离干/祖细胞
check_url/61126?article_type=v
隔离和卫星细胞的表征鼠头Branchiomeric肌肉
check_url/61126?article_type=v
从人多能干细胞的黑素细胞的无饲养推导
check_url/61126?article_type=v
分离和斑马鱼胚胎单细胞的表征
check_url/61126?article_type=v
小鼠胚胎Hemogenic内皮细胞的分离
check_url/61126?article_type=v
从人胎盘组织源分离和间充质干扩张/基质细胞
check_url/61126?article_type=v
人脐血内皮祖细胞的分离
check_url/61126?article_type=v
原发性大鼠瓣膜间质细胞的分离与鉴定: 主动脉瓣钙化的新模型研究
Read Article