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从鼠心心室自由壁分离内膜细胞和冠状内皮细胞
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
DOI:

08:22 min

April 15, 2020

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Chapters

  • 00:04Introduction
  • 01:11Heart Isolation
  • 02:34Endocardial Endothelial Cell (EEC) Digestion
  • 03:34Coronary Endothelial Cell (CEC) Digestion
  • 04:36Endothelial Cell (EC) Sorting
  • 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
  • 07:46Conclusion

Summary

Automatic Translation

我们提出了一种方案,通过消化缓冲液中的连续组织消化、循环离心循环的细胞采集以及使用抗大鼠CD31微珠进行细胞纯化,从大鼠心脏分离内皮和冠状内皮细胞。

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