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Isolamento das Células Endocárdicas e Coronárias da Parede Livre Ventricular do Coração de Rato
This content is Free Access.
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center
Chapters
- 00:04Introduction
- 01:11Heart Isolation
- 02:34Endocardial Endothelial Cell (EEC) Digestion
- 03:34Coronary Endothelial Cell (CEC) Digestion
- 04:36Endothelial Cell (EC) Sorting
- 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
- 07:46Conclusion
Apresentamos um protocolo para o isolamento de células endocárdicas e coronárias de corações de ratos através da digestão sequencial de tecidos em um tampão de digestão, coleta celular de ciclos de centrífuga recorrentes e purificação celular usando microesferas CD31 anti-ratos.
