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Genetics
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甲基化RNA免疫沉淀分析研究m
5C
在阿拉伯的修改
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Methylated RNA Immunoprecipitation Assay to Study m
5
C Modification in Arabidopsis
Please note that all translations are automatically generated.
Click here for the English version.
甲基化RNA免疫沉淀分析研究m
5C
在阿拉伯的修改
DOI:
10.3791/61231-v
•
08:50 min
•
May 14, 2020
•
Eleftheria Saplaoura*
1
,
Valentina Perrera*
2
,
Vincent Colot
,
Friedrich Kragler
1
Max Planck Institute of Molecular Plant Physiology
,
2
Department of Molecular Medicine, Medical School
,
University of Padua
,
3
Biologie de l’Ecole Normale Supérieure (IBENS), Paris, France
Chapters
00:04
Introduction
00:32
RNA Preparation
03:44
Methylated RNA Immunoprecipitation (MeRIP)
07:02
Results: Representative MeRIP Analyses
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
甲基化RNA免疫沉淀检测是一种基于抗体的方法,用于丰富甲基化RNA片段。再加上深度测序,它导致识别承载m
5
C修改的抄本。
Tags
Methylated RNA Immunoprecipitation
MeRIP
RNA Modification
Transcriptome
Arabidopsis
RNA Extraction
Guanidine Thiocyanate
Acid Phenol
1-bromo-3-chloropropane
Isopropanol
Sodium Acetate
Ethanol
DNase
RNA Integrity Number
In Vitro Transcript
Sonication
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