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Vaskulær støbning af voksne og tidlige postnatal mus lunger til Micro-CT Imaging
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1Translational Vascular Medicine Branch, National Heart Lung and Blood Institute,National Institutes of Health, 2Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 3Division of Pediatric Surgery, Department of Surgery,University of North Carolina at Chapel Hill
Chapters
- 00:04Introduction
- 00:52Pulmonary Artery (PA) Catheterization and Blood Perfusion
- 03:18Tracheostomy and Lung Inflation
- 04:59Casting the Vasculature
- 06:44Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
- 08:24Conclusion
Formålet med denne teknik er ex vivo visualisering af lungearteriale netværk af tidlige postnatal og voksne mus gennem lungeinflation og injektion af en radio-uigennemsigtig polymer-baseret forbindelse via lungearterien. Potentielle anvendelser for støbt væv er også drøftet.
