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形態学およびプロテオーム解析のための胚性子宮頸部神経節からのラット交感神経の培養
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
DOI:

14:51 min

September 27, 2020

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Chapters

  • 00:00Introduction
  • 00:09Preparation of Media for Dissection and for Culturing Neurons
  • 00:42Preparation of Plates for Culturing Neurons
  • 01:56Dissection Set-up
  • 02:36Embryo Removal and Preparation
  • 04:17Isolation of the SCG from the Embryo
  • 06:52Post-processing of SCG
  • 11:00Maintenance of the Cultured SCG Neurons and Treatments
  • 13:02Representative Results- Immunostaining
  • 14:00Representative Results- Proteomic Analysis
  • 14:29Conclusion

Summary

Automatic Translation

本論文では、上方の頸神経節からの胚性ラット交感神経の単離と培養について述べている。また、免疫細胞化学染色や質量分析のためのニューロン抽出物の準備のための詳細なプロトコルも提供します。

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