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Imagens Intranuclear Actin Rods em Embriões de Drosophila estressados de calor vivo

Article DOI: 10.3791/61297-v • 07:57 min • May 15th, 2020
May 15th, 2020 •
1,2, 3, 1,2,3
1Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

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O objetivo deste protocolo é injetar actina globular conjugada por Rhodamine em embriões Drosophila e montagem de haste de actina intranuclear de imagem após estresse térmico.

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Biologia do Desenvolvimento Edição 159 Drosophila Embrião G-actin Microinjeção Actin Stress Response Intranuclear Actin Rods FRAP Confocal Microscopy
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