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Native Cell Membrane Nanopartikler System for Membran Protein-Protein Interaktion Analyse
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Biochemistry
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JoVE Journal Biochemistry
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanopartikler System for Membran Protein-Protein Interaktion Analyse

DOI:
10.3791/61298-v

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07:31 min

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July 16, 2020

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, , , ,
1Department of Medicinal Chemistry, School of Pharmacy,Virginia Commonwealth University, 2Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy,Virginia Commonwealth University

Chapters

  • 00:05Introduction
  • 00:54Native Cell Membrane Nanoparticle (NCMN) Preparation
  • 03:04Negative Stain Grid Preparation
  • 04:55Results: Representative Negative Stain and Electrophoresis Gel images of Purified Acriflavine Resistance B (AcrB) Constructs
  • 06:52Conclusion

Summary

Automatic Translation

Automatic Translation

Præsenteret her er en protokol til bestemmelse af oligomerisk tilstand af membranproteiner, der bruger et indfødt cellemembran nanopartikelsystem i forbindelse med elektronmikroskopi.

Tags

Keywords: Native Cell Membrane NanoparticlesMembrane Protein-protein Interaction AnalysisElectron MicroscopyMembrane Protein StructureNative EnvironmentHigh-resolution Structure DeterminationMembrane Active PolymerNickel NTA ColumnFast Protein Liquid ChromatographyNegative Stain Grids

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