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Stabilitet og struktur av Bat Major Histocompatibility Complex klasse I med heterologøse β2-Microglobulin
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1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences,University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences, 5Savaid Medical School,University of Chinese Academy of Sciences
Chapters
- 00:04Introduction
- 00:54E. coli Transformation
- 01:46Culture Inoculation
- 02:59Bacteria Harvest
- 03:28Inclusion Body Purification
- 04:27MHC Complex Refolding
- 04:53MHC H Chain and Beta2m Injection and Dilution
- 05:42Refolded Protein Concentration Determination
- 06:51Crystallization, Data Collection, and Processing
- 07:41Structure Determination and Analyses
- 08:33Results: Representative Bat MHC I Beta2-Microglobulin Stability and Structure Analysis
- 10:21Conclusion
Protokollen beskriver eksperimentelle metoder for å oppnå stabile store histokompatibilitetskompleks (MHC) klasse I gjennom potensielle β 2-mikroglobulin (β2m) substitusjoner fra forskjellige arter. Den strukturelle sammenligningen av MHC I stabilisert av homolog og heterolog β2m ble undersøkt.
