Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

DOI:

10.3791/61476-v

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08:50 min

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June 12, 2020

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Deirdre M. Logsdon, Rebecca A. Kile, William B. Schoolcraft, Rebecca L. Krisher, Ye Yuan

¹Colorado Center for Reproductive Medicine

Chapters

00:04Introduction
01:24Preparation and Zona Removal
03:56Blastocyst Extended Culture
05:21Single Cell Selection and Sample Collection
06:33Results: Morphologies and Trophoblast Marker Expression during Extended Culture
07:59Conclusion

Summary

Automatic Translation

Here, we describe a method for warming vitrified human blastocysts, culturing them through the implantation period in vitro, digesting them into single cells and collecting early trophoblast cells for further investigation.

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

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Chapters

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