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Enkeltcellesamling af trophoblastceller i peri-implantationsstadium menneskelige embryoner
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JoVE Journal Biology
Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Enkeltcellesamling af trophoblastceller i peri-implantationsstadium menneskelige embryoner

DOI:
10.3791/61476-v

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08:50 min

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June 12, 2020

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, , , ,
1Colorado Center for Reproductive Medicine

Chapters

  • 00:04Introduction
  • 01:24Preparation and Zona Removal
  • 03:56Blastocyst Extended Culture
  • 05:21Single Cell Selection and Sample Collection
  • 06:33Results: Morphologies and Trophoblast Marker Expression during Extended Culture
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en metode til opvarmning af forglasificerede menneskelige blastocysts, der dyrker dem gennem implantationsperioden in vitro, fordøjer dem i enkelte celler og indsamler tidlige trophoblastceller til yderligere undersøgelse.

Tags

Keywords: Single Cell CollectionTrophoblast CellsPeri-implantation StageHuman EmbryosEarly Pregnancy LossRecurrent Implantation FailurePlacenta PathologiesIn Vitro CultureExtended Culture SystemEarly Trophoblast DifferentiationEmbryo WarmingFibronectinIVC 1 Media

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